cd79a pe  (Novus Biologicals)

Journal: BMC Veterinary Research

Article Title: Diagnosis of canine B-cell chronic lymphoid leukemia with a CD21 negative phenotype using the LT21 clone CD21 antibody in flow cytometry: a case report

doi: 10.1186/s12917-024-04335-x

Figure Lengend Snippet: Antibodies used for flow cytometry

Article Snippet: CD79a monoclonal antibody-PE (Mouse IgG1, κ) , HM47 , Invitrogen , 12-0792-42.

Techniques: Staining, Control, Negative Control

Journal: BMC Veterinary Research

Article Title: Diagnosis of canine B-cell chronic lymphoid leukemia with a CD21 negative phenotype using the LT21 clone CD21 antibody in flow cytometry: a case report

doi: 10.1186/s12917-024-04335-x

Figure Lengend Snippet: Amounts of antibodies used for flow cytometry analysis

Article Snippet: CD79a monoclonal antibody-PE (Mouse IgG1, κ) , HM47 , Invitrogen , 12-0792-42.

Techniques: Flow Cytometry, Staining, Control, Negative Control

Journal: BMC Veterinary Research

Article Title: Diagnosis of canine B-cell chronic lymphoid leukemia with a CD21 negative phenotype using the LT21 clone CD21 antibody in flow cytometry: a case report

doi: 10.1186/s12917-024-04335-x

Figure Lengend Snippet: Immunophenotyping of peripheral mononuclear cells before chemotherapy. Peripheral blood mononuclear cells isolated from the canine patient were immunophenotyped by flow cytometric analysis using FITC-conjugated mouse anti-dog CD3/PE-conjugated mouse anti-CD79A, FITC-conjugated rat anti-dog CD4/PE-conjugated rat anti-dog CD8 monoclonal antibody, FITC-conjugated mouse anti-dog CD21/PE-conjugated mouse anti-CD79A, PE-conjugated mouse anti-canine CD34, FITC-conjugated rat ant-canine CD45, FITC-conjugated rat anti-canine MHC class II, and FITC-conjugated mouse anti-human CD14 antibodies. Nonspecific binding of targeted antibodies to cell surface antigens was estimated using isotype control antibodies. Gating was performed with reference to populations of unstained, single stained, and isotype controls. FITC: fluorescein isothiocyanate; PE: phycoerythrin

Article Snippet: CD79a monoclonal antibody-PE (Mouse IgG1, κ) , HM47 , Invitrogen , 12-0792-42.

Techniques: Isolation, Binding Assay, Control, Staining

Journal: Leukemia

Article Title: The EGR3 regulome of infant KMT2A -r acute lymphoblastic leukemia identifies differential expression of B-lineage genes predictive for outcome

doi: 10.1038/s41375-023-01895-z

Figure Lengend Snippet: Patient characteristics of the dx cohort ( n = 50).

Article Snippet: PE Mouse Anti-Human CD79a Monoclonal Antibody , BD , BD Biosciences Cat# 563777, RRID: AB_2738423.

Techniques:

Journal: Leukemia

Article Title: The EGR3 regulome of infant KMT2A -r acute lymphoblastic leukemia identifies differential expression of B-lineage genes predictive for outcome

doi: 10.1038/s41375-023-01895-z

Figure Lengend Snippet: Patient characteristics of the rel cohort ( n = 18).

Article Snippet: PE Mouse Anti-Human CD79a Monoclonal Antibody , BD , BD Biosciences Cat# 563777, RRID: AB_2738423.

Techniques:

Journal: Leukemia

Article Title: The EGR3 regulome of infant KMT2A -r acute lymphoblastic leukemia identifies differential expression of B-lineage genes predictive for outcome

doi: 10.1038/s41375-023-01895-z

Figure Lengend Snippet: Oligonucleotides used in this study.

Article Snippet: PE Mouse Anti-Human CD79a Monoclonal Antibody , BD , BD Biosciences Cat# 563777, RRID: AB_2738423.

Techniques: Sequencing

Journal: Leukemia

Article Title: The EGR3 regulome of infant KMT2A -r acute lymphoblastic leukemia identifies differential expression of B-lineage genes predictive for outcome

doi: 10.1038/s41375-023-01895-z

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: PE Mouse Anti-Human CD79a Monoclonal Antibody , BD , BD Biosciences Cat# 563777, RRID: AB_2738423.

Techniques:

Journal: Leukemia

Article Title: The EGR3 regulome of infant KMT2A -r acute lymphoblastic leukemia identifies differential expression of B-lineage genes predictive for outcome

doi: 10.1038/s41375-023-01895-z

Figure Lengend Snippet: A Gating strategy. Single cells were gated out of cells of interest for assessment of CD19 and CD79A surface expressions. Analyses were performed based on four biological replicates. B Exemplary histogram of CD19 fluorescence intensities of a full-fluorescence-minus-anti CD19 (FMO) control (blue), SEM::mock (red) and SEM::EGR3 (orange). Plots display the respective single cell populations and were normalized to mode. C CD19 median fluorescence intensities (MFI) of SEM::mock and SEM::EGR3 singlets. Significance was tested using a two-tailed t test ( p < 0.0001). Error bars indicate standard deviation. D Mean percentages of the CD19 hi CD79A + population relative to singlets. Error bars indicate standard deviation and significance was tested using a two-tailed t test ( p = 0.0006). E CD79A median fluorescence intensities (MFI) of the CD19 hi CD79A + populations of SEM::mock and SEM::EGR3. Significance was tested using a two-tailed t test ( p = 0.0008). Error bars indicate standard deviation.

Article Snippet: PE Mouse Anti-Human CD79a Monoclonal Antibody , BD , BD Biosciences Cat# 563777, RRID: AB_2738423.

Techniques: Fluorescence, Two Tailed Test, Standard Deviation

Journal: Leukemia

Article Title: The EGR3 regulome of infant KMT2A -r acute lymphoblastic leukemia identifies differential expression of B-lineage genes predictive for outcome

doi: 10.1038/s41375-023-01895-z

Figure Lengend Snippet: Genes of the set ‘signaling by the B cell receptor’ (Reactome, R-HSA-983705), their type of regulation by EGR3 and differential expression.

Article Snippet: PE Mouse Anti-Human CD79a Monoclonal Antibody , BD , BD Biosciences Cat# 563777, RRID: AB_2738423.

Techniques: Expressing

Journal: Leukemia

Article Title: The EGR3 regulome of infant KMT2A -r acute lymphoblastic leukemia identifies differential expression of B-lineage genes predictive for outcome

doi: 10.1038/s41375-023-01895-z

Figure Lengend Snippet: Pearson correlation matrix indicating the Pearson r value ( A ) and p value ( B ) of all tested correlations. Volcano plots showing Pearson correlations of EGR3 ( C ), IGHM ( F ), BLK ( G ), CD79A ( J ), PTPN6 ( K ) and patient age ( L ). Correlations with p ≥ 0.05 were considered non-significant and marked in red. Patients plotted according to their IGHM / EGR3 ( D ), BLK / EGR3 ( E ), CD79A / EGR3 ( H ) and PTPN6 / EGR3 ( I ) ΔC T mean expression values.

Article Snippet: PE Mouse Anti-Human CD79a Monoclonal Antibody , BD , BD Biosciences Cat# 563777, RRID: AB_2738423.

Techniques: Expressing

Journal: Leukemia

Article Title: The EGR3 regulome of infant KMT2A -r acute lymphoblastic leukemia identifies differential expression of B-lineage genes predictive for outcome

doi: 10.1038/s41375-023-01895-z

Figure Lengend Snippet: A PCA of the diagnosis cohort among the IGHM , CD79A , BLK , and PTPN6 expressions. PC1 represents 82.4% and PC2 8.4% of total variance, respectively. B Heatmap of the PCA visualizing bimodal clustering of patients. C Kaplan–Meier curves and log-rank test of the BCR lo (red) and BCR hi (blue) patients. S.E.M. of each curve is indicated in bright color. D PCA of the diagnosis cohort among the IGHM , CD79A , BLK, and PTPN6 expressions with patients assigned to the age groups 0–6 months and 6–12 months. E Heatmap of the PCA visualizing bimodal clustering of patients is not affected by patient age at diagnosis. F PCA of the diagnosis cohort among the IGHM , CD79A , BLK, and PTPN6 expressions with patients assigned to HOXA status groups HOXA9 high (hi) and low (lo). G Heatmap of the PCA visualizing bimodal clustering of patients is not affected by HOXA status.

Article Snippet: PE Mouse Anti-Human CD79a Monoclonal Antibody , BD , BD Biosciences Cat# 563777, RRID: AB_2738423.

Techniques:

Journal: Leukemia

Article Title: The EGR3 regulome of infant KMT2A -r acute lymphoblastic leukemia identifies differential expression of B-lineage genes predictive for outcome

doi: 10.1038/s41375-023-01895-z

Figure Lengend Snippet: A PCA of the relapse cohort among the IGHM , CD79A , BLK, and PTPN6 expressions. PC1 represents 81.3% and PC2 12.3% of total variance, respectively. B Heatmap of the PCA visualizing clustering of patients. Pearson correlation matrix indicating the Pearson r value ( C ) and p value ( D ) of all tested correlations. Volcano plots showing Pearson correlations of IGHM ( E ), BLK ( F ), CD79A ( G ), PTPN6 ( H ) EGR3 ( I ) and patient age ( J ). Correlations with p ≥ 0.05 were considered non-significant and marked in red. Comparison of the BCR lo and BCR hi groups of the diagnosis and relapse cohorts regarding EGR3 relative expression ( K ), patient age ( L ), IGHM ( M ), CD79A ( N ), BLK ( O ) and PTPN6 ( P ) relative expressions. Bars indicate the median and 95% confidence interval.

Article Snippet: PE Mouse Anti-Human CD79a Monoclonal Antibody , BD , BD Biosciences Cat# 563777, RRID: AB_2738423.

Techniques: Expressing